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KMID : 0545120180280071141
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Journal of Microbiology and Biotechnology
2018 Volume.28 No. 7 p.1141 ~ p.1146
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A Simple Enzymatic Method for Quantitation of 2’-Fucosyllactose
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Seydametova Emine
Shin Jong-Hyeok
Yu Ji-Won
Kweon Dae-Hyuk
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Abstract
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2′-Fucosyllactose (2′-FL) is one of the most important human milk oligosaccharides and has several health benefits for infants. The levelsof 2′-FL in breast milk or samples from other sources can bequantified byhigh-performance liquid chromatography. However, this methodcannot be used for simultaneous detection of the target compound in numerous samples. Here, we developed a simplemethod for quantifying 2′-FL in a microplate format. The method involvestwosteps: (1) release of L-fucose from 2′-FL by α-(1-2,3,4,6)-L-fucosidase and (2) measurement ofNADPHformed during oxidation of L-fucose by L-fucose dehydrogenase. This method enablesmeasurement of up to 5 g/L2′-FL in 50minusing a 96-well microplate. The efficiencyand simplicity of the proposed method makes it suitable for the analyses of a large number of samples simultaneously.
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KEYWORD
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2′-Fucosyllactose, quantitation, α-(1-2,3,4,6)-L-fucosidase, L-fucose, L-fucose dehydrogenase
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